Journal: Frontiers in Aging Neuroscience

Article Title: Increased tau-induced inflammatory responses are associated with a greater degree of atherosclerosis in progressive supranuclear palsy

doi: 10.3389/fnagi.2025.1608631

Figure Lengend Snippet: Greater atherosclerotic burden and elevated plasma total tau, p-tau181 levels in patients with PSP. Using carotid artery Doppler ultrasound to assess the degree of carotid artery atherosclerosis by evaluating (A) max-CIMT, (B) max-CPT, (C) TPN, (D) CPS, and (E) carotid stenosis% in HCs ( n = 56) and patients with PSP ( n = 56). Using MRA to evaluate the degree of intracranial arterial atherosclerosis by recording (F) ICS vascular territory and (G) MRA GSS in HCs ( n = 56) and patients with PSP ( n = 56). (H) Heatmap of multivariable linear regression heatmap showing the association between disease duration/clinical scores H&Y staging scale, UPDRS-III, PSPRS, MMSE and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). Peripheral (I–L) blood lipid, (M) glycosylated hemoglobin, (N) homocysteine parameters, and plasma levels of (O) total tau, (P) p-tau181, (Q) p-tau396 in HCs ( n = 56) and patients with PSP ( n = 56). (R) Heatmap of multivariable linear regression heatmap showing the association between tau levels (total tau, p-tau181, p-tau396) and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). Results are expressed as mean and SD. Statistical approaches: (A–E, G, I–Q) covariance (ANCOVA), with age, gender, smoking status and stroke history as covariates, (F) chi-square test, (H, R) multivariable linear regression. In heatmaps, each cell represents the standardized regression coefficient (β) derived from multivariable linear regression models, where each Y -axis was regressed on X -axis while adjusting for age, gender, smoking history, hypertension, diabetes, and dyslipidemia (For MMSE, one more correction for period of education). The color intensity of heatmap indicates the strength and direction of association (β), with positive associations shown in red and negative in blue. P < 0.05 indicates statistical significance (* p < 0.05, ** p < 0.01, *** p < 0.001), and ns indicates p ≥ 0.05.

Article Snippet: The following ELISA kits were used: Human interleukin-6 (IL-6) ELISA kit (E-HSEL-H0003, Elabscience Biotechnology, Wuhan, China), Human interleukin-1β (IL-1β) ELISA kit (E-HSEL-H0001, Elabscience Biotechnology, Wuhan, China), Human interleukin-10 (IL-10) ELISA kit (E-EL-H0005, Elabscience Biotechnology, Wuhan, China), Human interferon γ (IFN-γ) ELISA kit (E-HSEL-H0007, Elabscience Biotechnology, Wuhan, China), Human tumor necrosis factor-α (TNF-α) ELISA kit (E-HSEL-H0109c, Elabscience Biotechnology, Wuhan, China), Human total tau ELISA kit (ml057755, mlbio, Shanghai, China),and Human P-tau181 ELISA kit (ml057691, mlbio, Shanghai, China), Human P-tau396 ELISA kit (E-EL-H5314c, Elabscience Biotechnology, Wuhan, China), Mouse interleukin-6 (IL-6) ELISA kit (ml098430, mlbio, Shanghai, China), Mouse interleukin-1β (IL-1β) ELISA kit (ml098416, mlbio, Shanghai, China) and Mouse tumor necrosis factor-α (TNF-α) ELISA kit (mIC50536-1, mlbio, Shanghai, China).

Techniques: Clinical Proteomics, Derivative Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Increased tau-induced inflammatory responses are associated with a greater degree of atherosclerosis in progressive supranuclear palsy

doi: 10.3389/fnagi.2025.1608631

Figure Lengend Snippet: Elevated peripheral blood macrophage ratio and plasma inflammatory cytokine levels in patients with PSP. Representative flow cytometry analysis of (A) macrophages (CD14 + CD68+), and (B) classically activated macrophages (CD86 + CD206-M1) subpopulations from peripheral blood (left) and quantifications (right) in HCs ( n = 10) and patients with PSP ( n = 10). Plasma levels of inflammatory cytokine (C) CRP, (D) IL-6, (E) IL-1β, (F) IL-10, (G) TNF-α, and (H) IFN-γ in HCs ( n = 56) and PSP with patients ( n = 56). (I) Heatmap of multivariable linear regression heatmap showing the association between inflammatory cytokine levels (CRP, IL-6, IL-1β, IL-10, TNF-α, and IFN-r) and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). (J) Heatmap of multivariable linear regression heatmap showing the association between tau levels (total tau, p-tau181, p-tau396) and inflammatory cytokine levels (CRP, IL-6, IL-1β, IL-10, TNF-α, and IFN-r) in patients with PSP ( n = 56). Results are expressed as mean and SD. Statistical approaches: (A, B) unpaired non-parametric Mann-Whitney U tests, (C–H) covariance (ANCOVA), with age, gender, smoking status and stroke history as covariates, (I, J) multivariable linear regression. In heatmaps, each cell represents the standardized regression coefficient (β) derived from multivariable linear regression models, where each Y -axis was regressed on X -axis while adjusting for age, gender, smoking history, hypertension, diabetes, and dyslipidemia. The color intensity of heatmap indicates the strength and direction of association (β), with positive associations shown in red and negative in blue. P < 0.05 indicates statistical significance (* p < 0.05, ** p < 0.01, *** p < 0.001), and ns indicates p ≥ 0.05.

Article Snippet: The following ELISA kits were used: Human interleukin-6 (IL-6) ELISA kit (E-HSEL-H0003, Elabscience Biotechnology, Wuhan, China), Human interleukin-1β (IL-1β) ELISA kit (E-HSEL-H0001, Elabscience Biotechnology, Wuhan, China), Human interleukin-10 (IL-10) ELISA kit (E-EL-H0005, Elabscience Biotechnology, Wuhan, China), Human interferon γ (IFN-γ) ELISA kit (E-HSEL-H0007, Elabscience Biotechnology, Wuhan, China), Human tumor necrosis factor-α (TNF-α) ELISA kit (E-HSEL-H0109c, Elabscience Biotechnology, Wuhan, China), Human total tau ELISA kit (ml057755, mlbio, Shanghai, China),and Human P-tau181 ELISA kit (ml057691, mlbio, Shanghai, China), Human P-tau396 ELISA kit (E-EL-H5314c, Elabscience Biotechnology, Wuhan, China), Mouse interleukin-6 (IL-6) ELISA kit (ml098430, mlbio, Shanghai, China), Mouse interleukin-1β (IL-1β) ELISA kit (ml098416, mlbio, Shanghai, China) and Mouse tumor necrosis factor-α (TNF-α) ELISA kit (mIC50536-1, mlbio, Shanghai, China).

Techniques: Clinical Proteomics, Flow Cytometry, MANN-WHITNEY, Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Decreasing β-Catenin Leads to Altered Endothelial Morphology, Increased Barrier Permeability and Cognitive Impairment During Chronic Methamphetamine Exposure

doi: 10.3390/ijms26041514

Figure Lengend Snippet: Elisa for p-Tau, NEFL(Nfl), and GFAP in serum of mice administered METH for 2 or 4 weeks. ( A ) Elisa for p-Tau in serum of mice post-administered METH. ( B ) Elisa for Nfl in serum of mice post-administered METH. ( C ) Elisa for GFAP in serum of mice post-administered METH. Data are expressed as mean ± SEM. Statistical significance was assessed using one-way ANOVA and Tukey HSD test (for multiple comparison test). The statistical significance is shown as ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Phosphorylated Tau (p-Tau), glial fibrillary acidic protein (GFAP) and neurofilament light polypeptide (Nfl/NEFL) in mice serum were measured by ELISA kits (p-Tau: E-EL-M1289; GFAP: E-EL-M0554; NEFL: E-EL-M0817; Elabscience, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Journal: Evolution; international journal of organic evolution

Article Title: Short-term heritable variation overwhelms two hundred generations of mutational variance for metabolic traits in Caenorhabditis elegans

doi: 10.1111/evo.14104

Figure Lengend Snippet: Adenosine metabolism pathway. Activity and concentration of enzymes Adenosine deaminase (ADA, red) and Adenosine kinase (ADK, red) were measured. We were unable to measure the concentration of APRT (pink). Metabolites in orange had concentrations quantified, those in gray were not measured.

Article Snippet: Enzyme activity assays: . (i) Adenosine kinase (ADK) Adenosine kinase (ADK) activity was measured using the Novocib PRECISE ADK assay kit (Novocib, Ref #K0507-01).

Techniques: Activity Assay, Concentration Assay

Journal: Evolution; international journal of organic evolution

Article Title: Short-term heritable variation overwhelms two hundred generations of mutational variance for metabolic traits in Caenorhabditis elegans

doi: 10.1111/evo.14104

Figure Lengend Snippet: Means. Column headings are: M 0 , G0 pseudoline mean/median (SEM); M MA , MA mean/median (SEM); R M , per-generation change in trait mean conditioned on extraction block (all traits) and total protein concentration (all traits except ADA activity and ADA and ADK concentration, (SEM); Δ M , per-generation change in trait mean scaled as a fraction of the G0 mean.

Article Snippet: Enzyme activity assays: . (i) Adenosine kinase (ADK) Adenosine kinase (ADK) activity was measured using the Novocib PRECISE ADK assay kit (Novocib, Ref #K0507-01).

Techniques: Extraction, Blocking Assay, Protein Concentration, Activity Assay, Concentration Assay

Journal: Evolution; international journal of organic evolution

Article Title: Short-term heritable variation overwhelms two hundred generations of mutational variance for metabolic traits in Caenorhabditis elegans

doi: 10.1111/evo.14104

Figure Lengend Snippet: Variances. Column headings are: V L,G0 , among-line variance of the G0 pseudolines (SEM); V L,MA , among-line variance of the MA lines (SEM); V E , the residual (within-line) variance of the MA lines (SEM); h M 2 ( n o P S ) , mutational heritability calculated as V L,MA /2 t V E ; h M 2 ( − P S ) , mutational heritability calculated as (V L,MA -V L,G0 )/2 t V E . See Methods for details of the estimation of variance components.

Article Snippet: Enzyme activity assays: . (i) Adenosine kinase (ADK) Adenosine kinase (ADK) activity was measured using the Novocib PRECISE ADK assay kit (Novocib, Ref #K0507-01).

Techniques: Activity Assay